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In order to solve the problems that software simulation cannot demonstrate the performance in real channel environment, and cannot measure the transmission rate and delay of real communication system, the implementation method of LTE communication system based on universal computer and universal software radio peripheral (USRP) is studied. The basic structure and principle of the system are analyzed. By combining general computer with the front end of software radio, the baseband processing functions are fulfilled by universal computers, while the transmission and receiving of radio signals are fulfilled by USRP, in addition, the open air interface (OAI) platform is used as software structure to realize data processing and system control. The software and hardware of the considered LTE system are configured, tested and verified in the scenario of 5MHz system bandwidth, where all functions of LTE communication system are realized. In addition, the multi-user access scenario including computer terminals and commercial terminals is further developed and realized. The test results show that the LTE communication system can fulfill interconnection with public Internet and maintain stable connection.