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The pneumatic single seed metering device and spoon chain metering device are affected by the high miss seeding rate, which leads to the poor detection effect of the system. In order to solve the above problems, a single-chip microcomputer detection system based on RS232 protocol is proposed. According to the hardware structure of single-chip microcomputer, the signal sensed by Hall sensor is transmitted to single-chip microcomputer by using single-chip microcomputer with RS-232 serial interface to obtain standard seed metering pulse. According to the RS232 protocol, the infrared photoelectric sensor circuit is designed to determine the specific location of the fault metering tube, and the sound and light alarm device is set to prevent the manager from hearing the alarm in the noisy environment and avoid the occurrence of missing broadcast. The process of single seed metering device particle seeding quality detection and missing seeding real-time detection was designed. The experimental results show that the system is consistent with the expected seeding effect, and the lowest miss seeding rate is 0.9%, which has good detection effect.