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Due to the error in the positioning parameters of current needle stick robots, the success rate of needle sticks is low, which causes the patient to endure many painful problems. A needle stick robot control system is designed. The system consists of a robot arm, a host computer control system, a high-definition laser image acquisition system, and a signal sensor. The main hardware part is selected DHT01 medical high-definition lens, PCI8814 image acquisition card, and a stronger ATMEGA32L single-chip microcomputer to ensure The sharpness of the collected pictures and the control precision of the robotic arm. The similarity of multi-frame images is calculated on the image sequence processing algorithm, the feature points of the images are extracted using the FAST operator, and the topological relationship of the image sequence is optimized based on the key frames to achieve accurate positioning of the target position. The experimental results show that the average deviation of the three-axis robot system design is 0.0449, 0.0383, and 0.0387, which approach the theoretical center position, and the success rate of one-time needle insertion can reach 94.50%.