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In the process of high-speed data acquisition, a large amount of data needs to be transmitted to the computer in real time. Through the research and analysis of various transmission methods, the data transmission using USB 3.0 transmission method is proposed, and the high-speed data transmission interface based on USB 3.0 is designed. One of the key technologies is to stabilize the speed of data transmission. Through the analysis of various USB 3.0 chips, the FT601 chip produced by FTDI is used. According to the corresponding data manual, the peripheral circuit of the FT601 chip is completed, and the field programmable gate array (FPGA) is used as the USB 3.0 transmission controller. The internal programming of the FPGA is performed using the Verilog language, and the data is buffered by using a first-in-first-out (FIFO) method, and the data exchange between the FT601 chip and the host computer is controlled and tested. The test results show that the interface can transmit data at an average rate of 350MB/s after corresponding configuration, ensuring the stability of data transmission speed and data integrity.