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In order to measure the fly speed of specimen, a novel measurement method based on multi-camera stereo vision technique is proposed. Firstly, we research the calibration method of multi-camera stereo vision measurement system. Patch matching is preformed to find the correspond points in each camera image. Finally, the three-dimensional coordinate and speed of the test point is calculated at each camera frame time. The result shows that the method can accurately measure the specimen fly speed with the advantages of flexible and simple operation