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Aimed at the high cost and long working time of the traditional microfluidic chip processing technology, a low-cost, 3D printer design scheme for microfluidic chip preparation is proposed. The design scheme is composed of a 3D printer and a host computer Control software. Wherein the host computer control software is responsible for analyzing and slicing the pre-built 3D model and generating the G-code format file; and the 3D printing control system responsible for receiving, analyzing G-code files and converting them into printer-recognizable control commands to complete rapid prototyping of objects. Described in detail the realization of the various functional modules of the 3D printer, gives the test printing result, confirmed that the printer has the advantages of low cost, high precision.