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Multi-channels serial data transmission is a new requirement for modern industrial control transmission, and the traditional industrial controller due to the limited number of interfaces, often difficult to meet the requirements. For multi-channel serial ports data collection and transmission of real needs, a data acquisition program based on FPGA for multi-channel serial ports was presented in this paper. The idea of simultaneous acquisition, buffering and packet data processing for multi-channels serial data, and the structure of hardware frame based on FPAG + multi-serials port + USB and bus different rate transmission software processing algorithm was introduced to achieve a multi-channel serial data collection and transmission, In order to verify the effectiveness of the design, a software radio platform as the experimental object was taken to design a 6-way serial transmission system in this paper. And finally through the transmission of the source data and the received data comparison found that the two consistent. The results show that the scheme was effective and the test meets the expected requirements, and achieved the purpose of multi-channel serial acquisition and transmission.