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In the Field Wired Communication System, the two-wire full-duplex Communication interface can synchronously transmit both the transmission signal and the received signal on a single pair wire. Consequently, it is difficult to collect the received signal. A new method of two-wire full-duplex interface testing is proposed based on receive/send conversion and adaptive echo offset technique. Firstly, a receive/send conversion circuit is designed to implement the isolation on the line transmission and reception. Secondly, Digital adaptive filtering techniques are used to eliminate line hybrid network balance impedance mismatch errors due to canceling the interference signal of near-end echo. Finally, an interface testing system is constructed based on software radio technology and storage testing technology. The results of simulation and experiments show that the method is feasible for interface testing of two-wire full-duplex communication.