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In order to meet the requirement of the video image processing for the digital camera with HD-SDI interface in electro-optical measuring system, an embedded image acquisition system based on HD-SDI interface is proposed. Combining with FPGA and the deserializer chip of TI Company implement conversion of serial data in HD-SDI format to a parallel image data to meet the needs of DSP and other processor input. FPGA extended peripheral memory to store and reorganize the image data and sent to DSP in a specific order. Image data is stored in DSP memory through DSP EMIF interface in DMA way, which realized the image data acquisition function to HD-SDI video. The system integrated HD-SDI data link equalization, decoding and data acquisition function, provided a glueless connection data source to DSP for further image processing.