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The purpose of this paper is to design a simulator with multi-channel serial communication interfaces to simulate the outputs of pressure sensors, which would be used for the test of closed-loop pressure control system. For the requirement of 72 transmission interfaces and 10 receiving interfaces, it would be more than 10 boards with the application of standard commercial communication cards, and the simulator would be large, complex, and difficult to achieve real-time and synchronous control among those channels. So, a multi-channel programmable I/O interface board is applied for RS-485 communication, whose protocol is programmed on the FPGA supported by LabVIEW platform; and regulation boards are deployed for signal’s isolated transmission and driving. All functions are implemented within a miniaturized portable device, which is flexible to simulate the signals based on the patterns set up by users,and has excellent performance during the test. The result shows the approach has strong versatility and adaptability for a variety of communication applications.