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Aiming at the work tedious repetitive and low efficiency in the installation and position of specimen during the high frequency fatigue crack test, the installation and position method of fatigue crack propagation specimen was proposed, which can recognize the center coordinates and radius of fixture and specimen automatically and quickly, measure the holes distance accurately. then the fuzzy PID was used to control the DC servo motor. In order to validate the accuracy of the fatigue crack propagation specimen installation and position system, the digital microscope was used to measure the specimen position deviation at different time points. The research results show that the proposed method can measure the holes distance between fixture and specimen accurately, the maximum deviation is 0.122mm. All above, the presented method is valid and accurate, which provides a valuable reference for other detecting and positioning systems based on machine vision., and it has theoretical significance and practical value.