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Aiming at the status quo of rotating machinery fault diagnosis intelligent degree is not high, using the visual language LabVIEW as the front-end development platform of the steam turbine fault diagnosis expert system, using MySQL as a backstage supporter's database of the system, developed a expert system of fault diagnosis for rotating machinery which based on production rule express knowledge.Introduced the overall structure,knowledge base and inference engine of the system.At last,the expert system is verified through instance and the experimental results show the practicability of the method.